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1996-03-04
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Document 0678
DOCN M9640678
TI HTLV infection among Italian intravenous drug users and North African
subjects detected by the polymerase chain reaction and serological
methods.
DT 9604
AU Colombo E; Magistrelli C; Mendozzi E; Cattaneo E; Achilli G; Ferrante P;
Institute of Medical Microbiology, Istituto di Ricovero e Cura a;
Carattere Scientifico (IRCCS), S. Maria Nascente, University of; Milan,
Italy.
SO J Med Virol. 1995 Sep;47(1):10-5. Unique Identifier : AIDSLINE
MED/96048315
AB Six hundred intravenous drug users (IVDUs) and two hundred North
Africans were screened for human T-cell leukemia virus (HTLV) antibodies
using several serological methods. Eighteen of the eighty-two
HTLV-seropositive individuals were also tested by the polymerase chain
reaction-DNA enzyme immunoassay (PCR-DEIA), a non-isotopic method of
immunoenzymatic detection of the amplified DNA. Of these eighteen
subjects, eight IVDUs were found to be HTLV-II-positive by the PCR-DEIA,
whereas all of the eighteen subjects were negative for HTLV-I. Western
blot (WB) confirmed six of the eight HTLV-positive subjects, while the
results of the remaining two were indeterminate. The results confirmed
the PCR-DEIA as a rapid and an efficient method of discriminating
between HTLV-I and HTLV-II infection, whereas serological tests,
including the WB, have limitations in terms of specificity and
sensitivity. Moreover, this study showed a higher frequency of HTLV
seroreactivity in the Italian IVDU population than in previous studies
and confirmed that HTLV-II is more frequent than HTLV-I in this
population.
DE Africa, Northern Base Sequence DNA, Viral/ANALYSIS Human
HTLV-I/GENETICS/IMMUNOLOGY HTLV-I Antibodies/BLOOD HTLV-I
Infections/COMPLICATIONS/*DIAGNOSIS/IMMUNOLOGY
HTLV-II/GENETICS/IMMUNOLOGY HTLV-II Antibodies/BLOOD HTLV-II
Infections/COMPLICATIONS/*DIAGNOSIS/IMMUNOLOGY Italy Molecular
Sequence Data Polymerase Chain Reaction Sensitivity and Specificity
Serodiagnosis Substance Abuse, Intravenous/*COMPLICATIONS Support,
Non-U.S. Gov't JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).